Effect of Kungiliya Vennai and Kalchunna Thailam on Excision Wound Healing in Albino Wistar Rats

Vishal Bhat; Arul Amuthan; Barizah Binti Mohamed Rosli; Nur Amirah Binti Khairuddin; Fatin Shahira Binti Md. Isa

Abstract

International Journal of Pharmacology and Clinical Sciences,2015,4,3,52-57.

DOI:10.5530/ijpcs.4.3.4

Published:Sept 2015

Type:Original Article

Authors:

Author(s) affiliations:

Vishal Bhat,¹ Arul Amuthan,¹ Barizah Binti Mohamed Rosli,² Nur Amirah Binti Khairuddin,²Fatin Shahira Binti Md. Isa²

¹Department of Pharmacology, Melaka Manipal Medical College and Center for Integrative Medicine and Research,Manipal University, Karnataka-576104, INDIA.

²MBBS Student, Melaka Manipal Medical College, Manipal University, Karnataka-576104, INDIA.

Abstract:

Background: Kungiliya vennai and Kalchunna thailam are the two indigenous herbal formulation being used for healing of wounds in siddha medicine. Objectives: The objective of our study was to evaluate the wound healing property of topical application of kungiliya vennai (KV) and kalchunna thailam (KT) on excision wound in Sprague Dawley rats. Methods: Four groups of eight Sprague dawley female rats in each group were used for the study. Under anesthesia, excision wound of 300mm2 size was created using scissors. Group 1 served as control with no drugs applied. Group 2, 3 and 4 were applied with mupirocin, KV and KT respectively daily until wound was completely healed. Parameters observed were % wound contraction rate and epithelialization period. Biopsy of wound for histology was taken on day 12 as well as the healed wound was taken after the wound was healed. Results were analyzed by one-way analysis of variance (ANOVA) followed by Tukey’s test by keeping p<0.05 as statistically significant. Results: Kalchunna thailam has increased wound contraction rate only during initial days, but not statistically significant. Kungiliya vennai treatment has increased vascularity with presence of tissue eosinophils when compared to control rats. There was a regeneration of adnexal skin structures in the Kungiliya vennai treated rats, which is not seen in other groups. The epithelialization period was significantly (p<0.001) hastened in Group 3 (15.83 days) and 4 (15.17 days) compared to control group (21.33 days). Conclusion: The topical application of kungiliya vennai and kalchunna thailam promoted wound healing in rats. Kungiliya vennai has the additional property of regenerating adnexal structures such as hair follicles, sweat glands and sebaceous glands.