Muscle Relaxant Property of 1(4-carboxy phenyl)-4,4,6-trimethyl-1H, 4H pyrimidine-2 thiol (a Pyrimidine Derivative) in Ex vivo Smooth and Skeletal Muscle Preparations

Background: Pyrimidine and its derivatives demonstrate a wide range of biological and pharmacological activities. Objective: To study the smooth muscle and skeletal muscle relaxant activity of a pyrimidine derivative 1 (4-carboxy phenyl)-4,4,6-trimethyl-1H,4H pyrimidine- 2 thiol (4CPTP), the chemical structure of which is similar to phenobarbitone. Material and Methods: Spasmolytic activity of 4CPTP was studied using rabbit ileum and guinea pig ileum preparation. Skeletal muscle relaxant activity was assessed on frog rectus abdominis preparation. Mean height of contractions with graded doses of phenobarbitone and 4CPTP along with mean inhibition of contractions were calculated. Statistical analysis was done using chi square test. Results: The test compound produced dose dependent decrease in acetylcholine and barium chloride induced contractions of rabbit ileum, histamine induced contractions of guinea pig intestine and acetyl choline induced contractions of frog rectus abdominis. Conclusion: 4 CPTP possesses spasmolytic and neuromuscular blocking activity in our ex vivo studies.